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Phosphate Buffered Solution/0.1% Tween (0.1% PBT)

NOTE: Procedure for 0.01% PBT can be found below.


Reagents (for 50 mL):

  • 25% Tween (Sigma-Aldrich Cat. No.9005-64-5) - 2mL
    • Note: The tween must be diluted to 25%.
  • 1X PBS – 48 mL

Materials:

  • 50 mL presterilised centrifuge tube (VWR Cat. No. 89004-364)

Procedure:

  1. Mix 200 μL of 25% Tween with 50 mL of 1X PBS.
  2. Mix thoroughly.

0.01% PBT

Reagents (for 50mL):

  • 25% Tween (Sigma-Aldrich Cat. No.9005-64-5) - 2mL
    • Note: The tween must be diluted to 25%.
  • 1X PBS – 48.8 mL

Materials:

  • 50 mL presterilised centrifuge tube (VWR Cat. No. 89004-364)

Procedure:

  1. Mix 20 μL of 25% Tween with 50 mL of 1X PBS.
  2. Mix thoroughly.

worm

Sudhir Nayak, Ph.D.

Biology Building 126
2000 Pennington Rd.
Ewing, NJ 08628
P) 609-771-2659

 

Dr. Nayak’s Lab
Biology Building 236
P) 609-771-3436
E) nayaklab@tcnj.edu